Publication

Nakata S, Iwasaki K, Funato H, Yanagisawa M, Ozaki H. Neuronal subtype-specific transcriptomic changes in the cerebral neocortex associated with sleep pressure. Neurosci Res 207:13-25, 2024

Sawada T, Iino Y, Yoshida K, Okazaki H, Nomura S, Shimizu C, Arima T, Juichi M, Zhou S, Kurabayashi N, Sakurai T, Yagishita S, Yanagisawa M, Toyoizumi T, Kasai H, Shi S. Prefrontal synaptic regulation of homeostatic sleep pressure revealed through synaptic chemogenetics. Science 385(6716):1459-1465, 2024

Okabe Y, Murakoshi N, Kurebayashi N, Inoue H, Ito Y, Murayama T, Miyoshi C, Funato H, Ishii K, Xu D, Tajiri K, Qin R, Aonuma K, Murakata Y, Song Z, Wakana S, Yokoyama U, Sakurai T, Aonuma K, Ieda M, Yanagisawa M. An inherited life-threatening arrhythmia model established by screening randomly mutagenized mice. Proc Natl Acad Sci USA 121:e2218204121, 2024

Cherasse Y, Taira Y, Rassu AL, Barateau L, Evangelista E, Muratani M, Funato H, Yanagisawa M, Dauvilliers Y. Association between idiopathic hypersomnia and a genetic variant in the PER3 gene. J Sleep Res e14146, 2024

Iwagami M, Seol J, Hiei T, Tani A, Chiba S, Kanbayashi T, Kondo H, Tanaka T, Yanagisawa M. Scientific Reports 13(1):21545, 2023

Miyanishi K, Hotta-Hirashima N, Miyoshi C, Hayakawa S, Kakizaki M, Kanno S, Ikkyu A, Funato H, Yanagisawa M. Neurosci Res S0168-0102(23)00205-5, 2023

Elhosainy A, Suzuki-Abe H, Kaushik MK, Kim SJ, Saitoh T, Ishikawa Y, Hotta-Hirashima N, Miyoshi C, Funato H, Yanagisawa M. Face validation and pharmacologic analysis of Sik3^Sleepy mutant mouse as a possible model of idiopathic hypersomnia. Eur J Pharmacol 954:175877, 2023

Asano F, Kim SJ, Fujiyama T, Miyoshi C, Hotta-Hirashima N, Asama N, Iwasaki K, Kakizaki M, Mizuno S, Mieda M, Sugiyama F, Takahashi S, Shi S, Hirano A, Funato H, Yanagisawa M. SIK3-HDAC4 in the suprachiasmatic nucleus regulates the timing of arousal at the dark onset and circadian period in mice. Proc Natl Acad Sci U S A 120(11): e2218209120, 2023

Kim SJ, Hotta-Hirashima N, Asano F, Kitazono T, Iwasaki K, Nakata S, Komiya H, Asama N, Matsuoka T, Fujiyama T, Ikkyu A, Kakizaki M, Kanno S, Choi J, Kumar D, Tsukamoto T, Elhosainy A, Mizuno S, Miyazaki S, Tsuneoka Y, Sugiyama F, Takahashi S, Hayashi Y, Muratani M, Liu Q, Miyoshi C, Yanagisawa M, Funato H. Kinase signalling in excitatory neurons regulates sleep quantity and depth. Nature 612(7940), 512-518, 2022

Zhou R, Wang G, Li Q, Meng F, Liu C, Gan R, Ju D, Liao M, Xu J, Sang D, Gao X, Zhou S, Wu K, Sun Q, Guo Y, Wu C, Chen Z, Chen L, Shi B, Wang H, Wang X, Li H, Cai T, Li B, Wang F, Funato H, Yanagisawa M, Zhang EE, Liu Q. A signaling pathway for transcriptional regulation of sleep amount in mice. Nature 612(7940), 519-527, 2022

Yamamoto H, Nagumo Y, Ishikawa Y, Irukayama-Tomobe Y, Namekawa Y, Nemoto T, Tanaka H, Takahashi G, Tokuda A, Saitoh T, Nagase H, Funato H, Masashi Yanagisawa. OX2R-selective orexin agonism is sufficient to ameliorate cataplexy and sleep/wake fragmentation without inducing drug-seeking behavior in mouse model of narcolepsy. PLoS One 17(7): e0271901, 2022

Fujiyama T, Takenaka H, Asano F, Miyanishi K, Hotta-Hirashima N, Ishikawa Y, Kanno S, Seoane-Collazo P, Miwa H, Hoshino M, Yanagisawa M, Funato H. Mice lacking cerebellar cortex and related structures show a decrease in slow-wave activity with normal non-REM sleep amount and sleep homeostasis. Front Behav Neurosci 16: 910461, 2022 

Suzuki-Abe H, Sonomura K, Nakata S, Miyanishi K, Mahmoud A, Hotta-Hirashima N, Miyoshi C, Sato TA, Funato H, Yanagisawa M. Metabolomic and pharmacologic analyses of brain substances associated with sleep pressure in mice. Neurosci Res 177: 16-24, 2022 

Choi J, Kim SJ, Fujiyama T, Miyoshi C, Park M, Suzuki-Abe H, Yanagisawa M, Funato H. The role of reproductive hormones in sex differences in sleep homeostasis and arousal response in mice. Front Neurosci 15: 739236, 2021 

Iwasaki K, Fujiyama T, Nakata S, Park M, Miyoshi C, Hotta-Hirashima N, Ikkyu A, Kakizaki M, Sugiyama F, Mizuno S, Abe M, Sakimura K, Takahashi S, Funato H, Yanagisawa M. Induction of mutant Sik3Sleepy allele in neurons in late infancy increases sleep need. Journal of Neuroscience 41(12): 2733–46, 2021

Kaushik MK, Aritake K, Cherasse Y, Imanishi A, Kanbayashi T, Urade Y, Yanagisawa M. Induction of narcolepsy-like symptoms by orexin receptor antagonists in mice. SLEEP 44(8): zsab043, 2021

Park M, Miyoshi C, Fujiyama T, Kakizaki M, Ikkyu A, Honda T, Choi J, Asano F, Mizuno S, Takahashi S, Yanagisawa M, Funato H. Loss of the conserved PKA sites of SIK1 and SIK2 increases sleep need. Scientific Reports 10: 8676, 2020

Hirose Y, Kitazono T, Sezaki M, Abe M, Sakimura K, Funato H, Handa H, Vogt K, Yanagisawa M. Hypnotic effect of thalidomide is independent of teratogenic ubiquitin/proteasome pathway. Proc Natl Acad Sci U S A 117(37):23106-12, 2020

Ogawa Y, Miyoshi C, Obana N, Yajima K, Hotta-Hirashima N, Ikkyu A, Kanno S, Soga T, Fukuda S, Yanagisawa M. Gut microbiota depletion by chronic antibiotic treatment alters the sleep/wake architecture and sleep EEG power spectra in mice. Scientific Reports 10(1):19554, 2020

Suzuki A, Yanagisawa M, Greene RW. Loss of Arc attenuates the behavioral and molecular responses for sleep homeostasis in mice. Proc Natl Acad Sci U S A 117(19):10547-53, 2020

Miyazaki T, Kanda T, Tsujino N, Ishii R, Nakatsuka D, Kizuka M, Kasagi Y, Hino H, Yanagisawa M. Dynamics of Cortical Local Connectivity during Sleep-Wake States and the Homeostatic Process. Cerebral Cortex 30(7):3977-90, 2020

Kakizaki M, Tsuneoka Y, Takase K, Kim SJ, Choi J, Ikkyu A, Abe M, Sakimura K, Yanagisawa M, Funato H. Differential roles of each orexin receptor signaling in obesity. iScience 20: 1-13, 2019

Miyoshi C, Kim SJ, Ezaki T, Ikkyu A, Hotta-Hirashima N, Kanno S, Kakizaki M, Yamada M, Wakana S, Yanagisawa M, Funato H. Methodology and theoretical basis of forward genetic screening for sleep/wakefulness in mice. Proc Natl Acad Sci USA 116(32): 16062-7, 2019

Honda T, Fujiyama T, Miyoshi C, Ikkyu A, Hotta-Hirashima N, Kanno S, Mizuno S, Sugiyama F, Takahashi S, Funato H, and Yanagisawa M. A single phosphorylation site of SIK3 regulates daily sleep amounts and sleep need in mice. Proc Natl Acad Sci U S A 115(41): 10458-63, 2018

Iwasaki K, Komiya H, Kakizaki M, Miyoshi C, Abe M, Sakimura K, Funato H, Yanagisawa M. Ablation of central serotonergic neurons decreased REM sleep and attenuated arousal response. Front Neurosci 12: 535, 2018

Fujiyama T, Miyashita S, Tsuneoka Y, Kanemaru K, Kakizaki M, Kanno S, Ishikawa Y, Yamashita M, Owa T, Nagaoka M, Kawaguchi Y, Yanagawa Y, Magnuson MA, Muratani M, Shibuya A, Nabeshima Y, Yanagisawa M, Funato H, Hoshino M. Forebrain Ptf1a is required for sexual differentiation of the brain. Cell Rep 24(1):79-94, 2018

Wang Z, Ma J, Miyoshi C, Li Y, Sato M, Ogawa Y, Lou T, Ma C, Gao X,Lee C, Fujiyama T, Yang X, Zhou S, Hotta-Hirashima N, Klewe-Nebenius D, Ikkyu A, Kakizaki M, Kanno S, Cao L, Takahashi S, Peng J, Yu Y, Funato H, Yanagisawa M, Liu Q. Quantitative phosphoproteomic analysis of the molecular substrates of sleep need. Nature 558(7710):435-9,2018

Kaushik MK, Aritake K, Imanishi A, Kanbayashi T, Ichikawa T, Shimizu T, Urade Y, Yanagisawa M. Continuous intrathecal orexin delivery inhibits cataplexy in a murine model of narcolepsy. Proc Natl Acad Sci U S A 115(23):6046-51, 2018

Komiya H, Miyoshi C, Iwasaki K, Hotta-Hirashima N, Ikkyu A, Kanno S, Honda T, Gosho M, Hamada H, Satoh T, Fukamizu A, Funato H, Yanagisawa M. Sleep/wakefulness behaviors in mice during pregnancy and pregnancy-associated hypertensive mice. SLEEP 41(3):  2018  doi: 10.1093/sleep/zsx209

Irukayama-Tomobe Y, Ogawa Y, Tominaga H, Ishikawa Y, Hosokawa N, Ambai S, Kawabe Y, Uchida S, Nakajima R, Saitoh T, Kanda T, Vogt K, Sakurai T, Nagase H, Yanagisawa M. Nonpeptide orexin type-2 receptor agonist ameliorates narcolepsy-cataplexy symptoms in mouse models. Proc Natl Acad Sci U S A 114(22):5731-6, 2017

Ogawa Y, Irukayama-Tomobe Y, Murakoshi N, Kiyama M, Ishikawa Y, Hosokawa N, Tominaga H, Uchida S, Kimura S, Kanuka M, Morita M, Hamada M, Takahashi S, Hayashi Y, Yanagisawa M. Peripherally administered orexin improves survival of mice with endotoxin shock. Elife 5: e21055, 2016

Funato H, Miyoshi C, Fujiyama T, Kanda T, Sato M, Wang Z, Ma J, Nakane S, Tomita J, Ikkyu A, Kakizaki M, Hotta-Hirashima N, Kanno S, Komiya H, Asano F, Honda T, Kim SJ, Harano K, Muramoto H, Yonezawa T, Mizuno S, Miyazaki S, Connor L, Kumar V, Miura I, Suzuki T, Watanabe A, Abe M, Sugiyama F, Takahashi S, Sakimura K, Hayashi Y, Liu Q, Kume K, Wakana S, Takahashi JS, Yanagisawa M. Forward-genetics analysis of sleep in randomly mutagenized mice. Nature 539(7629):378-83, 2016

Hossain MS, Asano F, Fujiyama T, Miyoshi C, Sato M, Ikkyu A, Kanno S, Hotta N, Kakizaki M, Honda T, Kim SJ, Komiya H, Miura I, Suzuki T, Kobayashi K, Kaneda H, Kumar V, Takahashi JS, Wakana S, Funato H, Yanagisawa M. Identification of mutations through dominant screening for obesity using C57BL/6 substrains. Sci Rep 6:32453, 2016

Motoike T, Long JM, Tanaka H, Sinton CM, Skach A, Williams SC, Hammer RE, Sakurai T, Yanagisawa M. Mesolimbic neuropeptide W coordinates stress responses under novel environments. Proc Natl Acad Sci U S A 113(21):6023-8, 2016

Lee IT, Chang AS, Manandhar M, Shan Y, Fan J, Izumo M, Ikeda Y, Motoike T, Dixon S, Seinfeld JE, Takahashi JS, Yanagisawa M. Neuromedin s-producing neurons act as essential pacemakers in the suprachiasmatic nucleus to couple clock neurons and dictate circadian rhythms. Neuron 85 (5): 1086-102, 2015

Ikeda Y, Kumagai H, Skach A, Sato M, Yanagisawa M. Modulation of circadian glucocorticoid oscillation via adrenal opioid-CXR7 signaling alters emotional behavior. Cell 155 (6): 1323-36, 2013

Suzuki A, Sinton CM, Greene RW, Yanagisawa M. Behavioral and biochemical dissociation of arousal and homeostatic sleep need influenced by prior wakeful experience in mice. Proc Natl Acad Sci U S A 110 (25): 10288-93, 2013

Chang I, Bramall AN, Baynash AG, Rattner A, Rakheja D, Post M, Joza S, McKerlie C, Stewart DJ, McInnes RR, Yanagisawa M. Endothelin-2 deficiency causes growth retardation, hypothermia, and emphysema in mice. J Clin Invest 123 (6): 2643-53, 2013

Funato H, Sato M, Sinton CM, Gautron L, Williams SC, Skach A, Elmquist JK, Skoultchi AI, Yanagisawa M. Loss of Goosecoid-like and DiGeorge syndrome critical region 14 in interpeduncular nucleus results in altered regulation of rapid eye movement sleep. Proc Natl Acad Sci U S A 107 (42): 18155-60, 2010

Matsuki T, Nomiyama M, Takahira H, Hirashima N, Kunita S, Takahashi S, Yagami K, Kilduff TS, Bettler B, Yanagisawa M, Sakurai T. Selective loss of GABA(B) receptors in orexin-producing neurons results in disrupted sleep/wakefulness architecture. Proc Natl Acad Sci U S A 106 (11): 4459-64, 2009

Irukayama-Tomobe Y, Tanaka H, Yokomizo T, Yanagisawa M, Sakurai T. Aromatic D-amino acids act as chemoattractant factors for human leukocytes through a G protein-coupled receptor, GPR109B. Proc Natl Acad Sci U S A 106 (10): 3930-4, 2009

Funato H, Tsai AL, Willie JT, Kisanuki Y, Williams SC, Sakurai T, Yanagisawa M. Enhanced orexin receptor-2 signaling prevents diet-induced obesity and improves leptin sensitivity. Cell Metab 9 (1): 64-76, 2009

Mieda M, Williams SC, Richardson JA, Tanaka K, Yanagisawa M. The dorsomedial hypothalamic nucleus as a putative food-entrainable circadian pacemaker. Proc Natl Acad Sci U S A 103 (32): 12150–5, 2006

Takayasu S, Sakurai T, Iwasaki S, Teranishi H, Yamanaka A, Williams SC, Iguchi H, Kawasawa YI, Ikeda Y, Sakakibara I, Ohno K, Ioka RX, Murakami S, Dohmae N, Xie J, Suda T, Motoike T, Ohuchi T, Yanagisawa M, Sakai J. A neuropeptide ligand of the G protein-coupled receptor GPR103 regulates feeding, behavioral arousal, and blood pressure in mice. Proc Natl Acad Sci U S A 103 (19): 7438-43, 2006

Sakurai T, Nagata R, Yamanaka A, Kawamura H, Tsujino N, Muraki Y, Kageyama H, Kunita S, Takahashi S, Goto K, Koyama Y, Shioda S, Yanagisawa M. Input of orexin/hypocretin neurons revealed by a genetically encoded tracer in mice. Neuron 46 (2): 297–308, 2005

Mieda M, Williams SC, Sinton CM, Richardson JA, Sakurai T, Yanagisawa M. Orexin neurons function in an efferent pathway of a food-entrainable circadian oscillator in eliciting food-anticipatory activity and wakefulness. J Neurosci 24 (46): 10493-501, 2004

Mieda M, Willie JT, Hara J, Sinton CM, Sakurai T, Yanagisawa M. Orexin peptides prevent cataplexy and improve wakefulness in orexin neuron-ablated model of narcolepsy in mice. Proc Natl Acad Sci U S A 101 (13): 4649-54, 2004

Beuckmann CT, Sinton CM, Williams SC, Richardson JA, Hammer RE, Sakurai T, Yanagisawa M. Expression of a poly-glutamine-ataxin-3 transgene in orexin neurons induces narcolepsy-cataplexy in the rat. J Neurosci 24 (18): 4469-77, 2004

Xiong Y, Miyamoto N, Shibata K, Valasek MA, Motoike T, Kedzierski RM, Yanagisawa M. Short-chain fatty acids stimulate leptin production in adipocytes through the G protein-coupled receptor GPR41. Proc Natl Acad Sci U S A 101 (4): 1045-50, 2004

Willie JT, Chemelli RM, Sinton CM, Tokita S, Williams SC, Kisanuki YY, Marcus JN, Lee C, Elmquist JK, Kohlmeier KA, Leonard CS, Richardson JA, Hammer RE, Yanagisawa M. Distinct narcolepsy syndromes in Orexin receptor-2 and Orexin null mice: molecular genetic dissection of Non-REM and REM sleep regulatory processes. Neuron 38 (5): 715-30, 2003

Yamanaka A, Beuckmann CT, Willie JT, Hara J, Tsujino N, Mieda M, Tominaga M, Yagami K, Sugiyama F, Goto K, Yanagisawa M, Sakurai T. Hypothalamic orexin neurons regulate arousal according to energy balance in mice. Neuron 38 (5): 701-13, 2003

Tanaka H, Yoshida T, Miyamoto N, Motoike T, Kurosu H, Shibata K, Yamanaka A, Williams SC, Richardson JA, Tsujino N, Garry MG, Lerner MR, King DS, O’Dowd BF, Sakurai T, Yanagisawa M. Characterization of a family of endogenous neuropeptide ligands for the G protein-coupled receptors GPR7 and GPR8. Proc Natl Acad Sci U S A 100 (10): 6251-6, 2003

Gariepy CG, Ohuchi T, Williams SC, Richardson JA, Yanagisawa M. Salt-sensitive hypertension in endothelin-B receptor-deficient rats. J Clin Invest 105 (7): 925-33, 2000

Chemelli RM, Willie JT, Sinton CM, Elmquist JK, Scammell T, Lee C, Richardson JA, Williams SC, Xiong Y, Kisanuki Y, Fitch TE, Nakazato M, Hammer RE, Saper CB, Yanagisawa M. Narcolepsy in orexin knockout mice: molecular genetics of sleep regulation. Cell 98 (4): 437-51, 1999

Sakurai T, Amemiya A, Ishii M, Matsuzaki I, Chemelli RM, Tanaka H, Williams SC, Richardson JA, Kozlowski GP, Wilson S, Arch JRS, Buckingham RE, Haynes AC, Carr SA, Annan RS, McNulty DE, Liu W.-S., Terrett JA, Elshourbagy NA, Bergsma DJ, Yanagisawa M. Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior. Cell 92 (4): 573-85, 1998

Baynash AG, Hosoda K, Giaid A, Richardson JA, Emoto N, Hammer RE, Yanagisawa M. Interaction of endothelin-3 with endothelin-B receptor is essential for development of epidermal melanocytes and enteric neurons. Cell 79 (7): 1277-85, 1994

Hosoda K, Hammer RE, Richardson JA, Baynash A, Cheung JC, Giaid A, Yanagisawa M. Targeted and natural (piebald-lethal) mutations of endothelin-B receptor gene produce megacolon associated with spotted coat color in mice. Cell 79 (7): 1267-76, 1994

Xu D, Emoto N, Giaid A, Slaughter C, Kaw S, deWit D, Yanagisawa M. ECE-1: a membrane-bound metalloprotease that catalyzes the proteolytic activation of big endothelin-1. Cell 78 (3): 473-85, 1994

Sakurai T, Yanagisawa M, Takuwa Y , Miyazaki H , Kimura  S, Goto K, Masaki T. Cloning of a cDNA encoding a non-isopeptide selective subtype of the endothelin receptor. Nature 348 (6303): 732-5, 1990

Yanagisawa M, Kurihara H, Kimura S, Tomobe Y, Kobayashi M, Mitsui Y, Yazaki Y, Goto K, Masaki T. A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature 332 (6163): 411-5, 1988